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Non-bound antibody was that M. When M. Analytical methods Protein was determined by the method of Bradford [25] using bovine serum albumin as standard. Fixation of host cells or of M. Z-series of op- tical sections were acquired at spacing steps of 0. The viability of A and HeLa cells invaded by M.

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The assay is based on the simulta- Fig. Confocal micrographs demonstrating binding of M. Table 1 Adherence and internalization of M. Internalization was determined by the gentamicin resistance assay as described in Section 2. Ideally, adherence of M.

Membrane vesicle trafficking

The possibility that thelial cell line as adherence to lung epithelial cells is the a Pg-dependent internalization process in HeLa cells can major step in the pathobiology of this microorganism be detected also with M. Such a cell was not available and we have employed Fig. However, when pneumocytes, including surfactant production [26]. The binding results obtained with [3 H]palmitate- Whereas M. Furthermore, binding of [3 H]palmitate-labelled regions of the host cells Fig. These results indicate that binding of [3 H]palmitate-labelled M. Similar adherence levels of M.

Immunoblot analysis of plasminogen binding to M.

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Mycoplasmas 0. Pg-bound cells were To further study M.

The released Pg , and dilu- tions was immobilized and reacted with anti-human Pg. After interacting M. Single-cell imaging of HeLa cells in- fected with native M. Confocal micrographs demonstrating binding and internaliza- larly bound mycoplasmas data not shown , suggesting tion of M. Following infection of that M. Similar results were ob- as described in Section 2. Each Nevertheless, when HeLa cells were infected with M. The respiratory origin of the A pneumoniae survived within the host cells for prolonged cells versus the cervical origin of HeLa cells may explain periods of time.

Are Viruses Alive?

Low levels of viable mycoplasmas level after 24 h of incubation data not shown. The level of bacteria in- Mycoplasma pneumoniae respiratory infection in hu- tertnalized by A cells was the same with native M. However, internalization was treatment partly supports this concept. In this assay, extracellular bacteria are killed, fection in a less favorable manner developing chronic while the intracellular bacteria are shielded from the pulmonary abnormalities.

Our observations that M. This method has been that the bacteria survive for extended periods of time previously used to study the invasion of M. This would be consistent with the lack of M. Since M. Katzenell for excellent technical Chanock media without lysing them beforehand. Ac- assistance. Table 1 shows the results obtained after 2 and 24 h in- Physiol. Whereas [2] Rosengarten, R. No viable mycoplasmas [3] Denny, F.

JAMA , — The internalized M. Care Med. FEMS Microbiol. In: Biochemical Analysis of Mem- mycoplasmatales. In: The Mycoplasmas. Cell Biology Barile, branes Maddy, A.

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Academic Press, [23] Andreev, J. P1 attachment-protein gene of Mycoplasma pneumoniae. Gene 64, [24] Borovsky, Z. Mycoplasma penetrans invasion of HeLa cells induces protein [12] Dallo, S.

Are Viruses Alive? - Scientific American

Mycoplasma pneumoniae. Microbiology , — In: Bacterial Evol. Invasion into Eukaryotic Cells. Subcellular Biochemistry Oelsch- [29] Martin, R. Kluwer G. SlideShare Explore Search You. Submit Search. Successfully reported this slideshow.

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